story 1012 de kaldi. 1083 1141 1189 125 0148004 One Plasma Cell and 1 Proerythroblast. Early erythroblasts have a deep blue cytoplasm, and a coarse nuclear chromatin similar to plasma cells, but differ in having a high nuclear/cytoplasmic ratio and a centrally located nucleus in a round shaped cell. Also, they usually lack cytoplasmic vacuoles. Normal marrow - 100X 0148005 Left frame 1 Mature Lymphocyte and one Proerythroblast. The lymphocyte is small, has a high nuclear/cytoplasm ratio, gray-blue cytoplasm and a smudgy chromatin pattern. The proerythroblast is medium-large in size with a high nuclear/cytoplasmic ratio, basophilic blue cytoplasm with a small colorless area adjacent to the nucleus where the Golgi, centrisome and mitochondria are located, and a semi dense uniformly distributed nuclear chromatin pattern. Nucleoli may be evident. Center frame One Plasma Cell (top) with an eccentrically located nucleus, blue-gray cytoplasm containing several vacuoles, and a semi dense nuclear chromatin showing randomly distributed less dense areas. The Basophilic Erythroblast beneath it has a cytoplasm of similar color but a very high nuclear/cytoplasm ratio, a centrally located nucleus with a chromatin pattern that is more evenly distributed as to dense and less dense aggregates. Right frame 2 Polychromatic NRBC and one Proerythroblast with features similar to the proerythroblast in the left frame. Idiopathic thrombocytopenic purpura. Marrow - 100X 0148011 Three Plasma Cells. One at left center is elliptical in shape with an eccentrically located nucleus, deep basophilic blue cytoplasm containing a clear area adjacent to the nucleus and several vacuoles. Its nuclear chromatic is dense with a few less dense areas scattered throughout. Below it is an oval shaped plasma cell with similar features except for the vacuoles. At top edge is a Binucleated Plasma Cell. Its nuclear chromatin is less dense than the other plasma cells. A progranulocyte is at the center. It is large with a high nuclear/cytoplasm ratio, blue cytoplasm with prominent red-purple granules (azurophilic, primary) granules and a nucleus with evenly distributed chromatin. Idiopathic thrombocytopenic purpura. Marrow - 100X 0148014 One Binucleated Plasma cell and three Orthochromatic NRBC. Similar to the plasma cell, late NRBC have dense nuclear chromatin. When they show open areas, they are more distinct and colorless than in plasma cells and more uniformly distributed. Normal marrow - 100X 0148015 One Binucleated Plasma Cell with a fragmenting cytoplasm, probably an artifact of smear preparation. One blast and several neutrophilic myelocytes and later neutrophilic cells are also in the field. Normal marrow - 100X 0148016 One Plasma Cell with Four Nuclei. Its Golgi area (light in color) is in its center. Four blasts surround it. They are medium in size, have a high nuclear/cytoplasmic ratio, blue cytoplasm and an evenly distributed nuclear chromatin with one or more prominent nucleoli. Acute myeloid leukemia. Marrow - 100X 0148017 Left frame: Tri-nucleated Plasma cell. Right frame: Plasma cell with cytoplasm stuffed with packets of immunoglobulin. Their blueness is a result of a staining solution that is basic in pH and/or their location in the otherwise blue cytoplasm. Four Blasts and one monocyte complete the field. Acute myeloid leukemia. Marrow - 100X 0148018 One Plasma Cell that is Dying, thus the very dense, pyknotic nuclear chromatin. Two late neutrophils are also in the field. Blood - 100X 0148019 Left frame: 1 Plasma cell containing multiple large blue staining granules and one NRBC (bottom) containing numerous small blue staining granules. This is an Iron Stain preparation and the blue granules are hemosiderin. The NRBC is a pathologic sideroblast since there are many positive granules which are localized in distribution. Right frame: 1 Proerythroblast with a high nuclear/cytoplasmic ratio, blue cytoplasm showing a colorless area where the Golgi, mitochondria and centrosome are located, and a semi-dense nuclear chromatin evenly distributed. Above it is a Plasma Cell with five large black granules in its cytoplasm. The left frame is stained with Wright's-Giemsa and the right frame with Potassium Ferrocyanide. Alcoholic with liver disease. Marrow - 100X 0148020 One large Macrophage (histiocyte) in center of many Plasma Cells. The macrophage is very large and has a highly vacuolated cytoplasm reflecting engulfed and processed material. Otherwise, its N/C ratio and nuclear chromatin density are similar to some plasma cells. Multiple myeloma. Marrow - 50X 0148021 One Plasma Cell (left), 1 Macrophage (center) and 3 lymphocytes. The macrophage has a fine, evenly distributed and lighter staining chromatin pattern versus the coarser, unevenly distributed and darker staining chromatin pattern in the plasma cell. The lymphocytes nuclei are also semi-dense and darker staining. Plasma cells have blue cytoplasm of various shades and may contain a few vacuoles. Macrophage cytoplasm is gray and often contains multiple vacuoles of variable size. Cellular outline of plasma cells is distinct, whereas macrophages have an irregular one. Normal marrow - 100X 0148029 One Plasmacytoid Lymphocyte with multiple clear staining crystals which represent stored crystalized immunoglobulin. Serum sickness. Blood - 100X 0148031 One Mott Cell (plasma cell with vacuoles). 1 Binucleated Early Megakaryocyte with an extensive Golgi area where early granulation is occurring. The nuclear chromatin is less dense than in plasma cells. Five mature lymphocytes, smudge nuclei and 2 late NRBC are in the field. One NRBC is at lower right of the Mott cell. Compare its nuclear chromatin and cytoplasmic color with the lymphocytes. Autoimmune hemolytic anemia. Marrow - 100X 0148032 Top frame: 1 large Plasma Cell, 2 lymphocytes, 1 late NRBC, 1 mature and 1 band neutrophil, 1 metamyelocyte and 1 myelocyte. Lower frame: 1 plasma cell with multiple vacuoles. It is sometimes called a Grape cell, a Morula cell or a Mott Cell. The vacuoles represent stored immunoglobulin and have been termed Russell Bodies. Also in the field are a promyelocyte, 2 myelocytes, 1 band neutrophil, 3 late NRBC with barely any cytoplasm and 2 blasts squeezed between the plasma cell and myelocyte.Idiopathic thrombocytopenic purpura. Marrow - 100X 0148033 One Plasma Cell with Multiple Vacuoles. The cell to its upper right has a very basophilic blue cytoplasm with 1 distinct vacuole and a colorless area encircling most of the nucleus. It resembles an early NRBC, but the dense nuclear chromatin and intermediate nuclear/cytoplasm ratio suggest it is a plasma cell. An early eosinophil is at top center. Several neutrophilic myelocytes, and bands are present. Normal marrow - 100X 0148034 One Plasma Cell with Multiple Vacuoles. Their blue color results from a staining solution with a basic pH. Numerous neutrophilic cells from myelocytes through mature stage are present. The numerous NRBC show defective hemoglobinization in the cytoplasm. Anemia of chronic infection. Marrow - 100X 0148035 One Plasma Cell with Multiple Vacuoles at top left, one Plasma Cell with Multiple Nuclei at its right, and one plasma cell with two nuclei beneath that cell. Several neutrophilic myelocytes, band and mature neutrophils and one basophil are also in the field. A mature megakaryocyte with a single nucleus is partially seen at left edge. Marrow - 100X 0148036 One Grape Cell - a plasma cell with multiple vacuoles which represent packets of stored immunoglobulin. These vacuoles may be colorless, blue or pink depending upon the pH of the stain solution. Marrow - 100X 0148037 Two large Plasma Cells; one with Multiple Vacuoles, two of which are over the nucleus. Myelodysplastic syndrome (MDS). Marrow - 100X 0148038 One Plasma Cell with a large Vacuole Overlying its Nucleus. Marrow - 100X 0148039 One Plasma Cell with a large Vacuole Overlying its Nucleus. Marrow - 100X 0148043 One Plasma Cell with some features of an Osteoblast. Its oval shape, eccentrically located nucleus and low nuclear/cytoplasmic ratio are features found in both cells. The deep basophilic blue cytoplasm with a distinct cellular outline is plasmacytic. Its light staining area (Golgi) touches the nucleus, typical of a plasma cell. The dense nuclear chromatin with randomly distributed lighter areas is typical of a plasma cell. The appearance of the nucleus extending beyond the cell's border is typical for an osteoblast. Normal marrow - 100X 0148044 Left frame: 1 Osteoblast with elongated shape, low nuclear/cytoplasm ratio, eccentrically located nucleus which extends beyond the cytoplasm border, a blue-gray cytoplasm with a distinct colorless area away from the nucleus, and an irregular cytoplasmic outline with some spiculation. The nuclear chromatin is more dense than usual. Right frame: Two Plasma Cells with oval shape, eccentrically located nuclei, basophilic blue cytoplasm with a pale staining area (Golgi) adjacent to the nucleus and a semi-dense nuclear chromatin. Compare with the osteoblast in the left frame. Two mature and one myelocyte stage neutrophils are in the field. Normal marrow - 100X 0148052 Red cell Agglutination. Most of the red cells are in amorphous clumps. Clumping of the red cells indicates a cold antibody disorder whereas rouleaux formation indicates a protein disorder. Cold agglutinin hemolytic anemia. Blood - 50X 2661007 Bare megakaryocyte nuclei. Normal marrow - 100X